Cybrydy

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Cybrys, cytoplasmic hybrids - cells obtained by biotechnological methods as a result of fusion of protoplasts. Hydrogens have the nucleus of one component and the cytoplasm of the other. In some cases, they may contain a hybrid cytoplasm that contains the mitochondria of one component and another of the plastics. Protoplast fusion is performed to transfer traits between two organisms when the parent plants are not capable of reproductive reproduction. The ability to resist disease, stress, herbicide. Getting

Cytoplasmic hybrids are the result of asymmetric fusion. Protoplasts of one of the components are treated with iodoacetamide, leading to destruction of the cytoplasm without affecting the nucleus. Protoplasts of the second component are exposed to X or UV radiation. Under the influence of radiation, the DNA contained in the cell nucleus is damaged. The protoplast fusion is carried out to transfer the characteristics of one organism to another. The protoplasts are collected in a solution of 10-50% polyethylene glycol. It is also possible to carry out electrofusion, which involves placing protoplasts between the electrodes. The fusion is caused by a short current of 0.5-4 MHz and a field strength of 50-1000 V cm

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