Klinger substrate

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Klinger substrate - a diagnostic solid substrate for gram-negative sticks with low nutritional requirements. Frequently used in the diagnosis of enterobacteria. It allows you to examine the ability of glucose, lactose and hydrogen sulphide production. The substrate is prepared in small semi-chunks. The medium contains peptone, lactose, glucose, ferrous sulphate and phenol red indicator. Glucose is expressed by acidification and yellowing of the entire medium. No change in the color of the substrate means no degradation of sugars. The production of large quantities of CO2 or H2 during fermentation is visualized by tearing the substrate or lifting it. The formation of H2S occurs in an acidic environment (the acidic environment is formed during the degradation of glucose) and results in the formation of an insoluble black iron sulfide precipitate. Bibliography Eligia Szewczyk: Bacteriological diagnostics.

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